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Santa Cruz Biotechnology
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Boster Bio
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Bethyl
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Image Search Results
Journal: Frontiers in Immunology
Article Title: Hydrogen Sulfide Reduces Myeloid-Derived Suppressor Cell-Mediated Inflammatory Response in a Model of Helicobacter hepaticus -Induced Colitis
doi: 10.3389/fimmu.2018.00499
Figure Lengend Snippet: H 2 S production is significantly reduced during Helicobacter hepaticus ( Hh )-induced colitis. 129SvEv Rag2 −/− mice were infected with Hh and sacrificed 6 weeks post-infection. (A) Representative western blot and (B) relative CSE and CBS mRNA expression performed on colon samples from uninfected and Hh -infected mice. A significant reduction in the expression of both CBS protein and mRNA was observed in Hh -infected mice. (C) H 2 S synthesis by the colon of healthy mice was measured in colon homogenates, in the absence or presence of CBS inhibitor [(CHH), 3 mmol/L] or CSE inhibitor (PAG, 10 mmol/L). The substrate for H 2 S synthesis ( l -cys) was present at 4 mmol/L. Only CHH significantly reduced H 2 S synthesis in tissue from healthy controls (*** P < 0.001 vs. l -cys alone). (D) H 2 S levels measured in the colon of Hh -infected mice 3 or 6 weeks post-infection resulted significantly reduced as compared to uninfected mice. Data are shown as mean ± SEM ( n = 5 per group) (** P < 0.01, *** P < 0.001 vs. uninfected).
Article Snippet: Then, the filters were incubated with the following primary antibodies: CSE (1:500 dilution, Proteintech),
Techniques: Infection, Western Blot, Expressing
Journal: Frontiers in Pharmacology
Article Title: Shensu IV maintains the integrity of the glomerular filtration barrier and exerts renal protective effects by regulating endogenous hydrogen sulfide levels
doi: 10.3389/fphar.2024.1447249
Figure Lengend Snippet: Primer sequence for RT-qPCR.
Article Snippet: Membranes were blocked with 5% skim milk (Solarbio) to prevent nonspecific binding and then incubated with primary antibodies against CD2AP (1:2000; A01756-2, BOSTER, Wuhan, China),
Techniques: Sequencing
Journal: Frontiers in Pharmacology
Article Title: Shensu IV maintains the integrity of the glomerular filtration barrier and exerts renal protective effects by regulating endogenous hydrogen sulfide levels
doi: 10.3389/fphar.2024.1447249
Figure Lengend Snippet: Shensu IV regulates the PI3K/AKT signaling pathway through H2S. (A) The effects of Shensu IV and NaHS on the mRNA expression of CD2AP, nephrin, CBS, CSE, NOX4, PI3K, and AKT in renal tissue of PAN rats were analyzed by RT-qPCR. (B) Western blot analysis of the effects of Shensu IV and NaHS on the protein levels of CD2AP, nephrin, CBS, CSE, NOX4, PI3K, p-PI3K,AKT,p-AKT in renal tissue of PAN rats. * P < 0.05, ** P < 0.01, *** P < 0.001. Abbreviations: CD2AP, CD2-associated protein; CBS, Cystathionine β-synthase; CSE, Cystathionine γ-lyase; PI3K, Phosphoinositide 3-Kinase; AKT, Protein Kinase B.
Article Snippet: Membranes were blocked with 5% skim milk (Solarbio) to prevent nonspecific binding and then incubated with primary antibodies against CD2AP (1:2000; A01756-2, BOSTER, Wuhan, China),
Techniques: Expressing, Quantitative RT-PCR, Western Blot
Journal: Frontiers in Pharmacology
Article Title: Shensu IV maintains the integrity of the glomerular filtration barrier and exerts renal protective effects by regulating endogenous hydrogen sulfide levels
doi: 10.3389/fphar.2024.1447249
Figure Lengend Snippet: Shensu IV regulates the PI3K/AKT signaling pathway through H2S in podocytes. (A) The effects of Shensu IV and NaHS on the mRNA expression of CD2AP, nephrin, CBS, CSE, NOX4, PI3K, and AKT in podocytes were analyzed by RT-qPCR. (B) Western blot analysis of the effects of Shensu IV and NaHS on the protein levels of CD2AP, nephrin, CBS, CSE, NOX4, PI3K, p-PI3K,AKT,p-AKT in PAN-induced podocyocytes. * P < 0.05, ** P < 0.01, *** P < 0.001. Abbreviations: CD2AP, CD2-associated protein; CBS, Cystathionine β-synthase; CSE, Cystathionine γ-lyase; PI3K, Phosphoinositide 3-Kinase; AKT, Protein Kinase B.
Article Snippet: Membranes were blocked with 5% skim milk (Solarbio) to prevent nonspecific binding and then incubated with primary antibodies against CD2AP (1:2000; A01756-2, BOSTER, Wuhan, China),
Techniques: Expressing, Quantitative RT-PCR, Western Blot
Journal: Cell metabolism
Article Title: Transsulfuration activity can support cell growth upon extracellular cysteine limitation
doi: 10.1016/j.cmet.2019.09.009
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Rabbit polyclonal anti-CBS ,
Techniques: Virus, Recombinant, SYBR Green Assay, Plasmid Preparation, Software, CRISPR